The Effects of Nonnutritive Sweeteners on the Cariogenic Potential of Oral Microbiome

Figure 3

Effects of NNSs on bacterial composition and extracellular polysaccharides (EPS)/acid production of saliva-derived multispecies biofilms. (a) Representative images of multispecies biofilms after fluorescence in situ hybridization. Green: S. mutans; red: S. sanguinis; blue: universal bacteria. (b) Biomass ratio quantitative data of S. mutans/universal bacteria (blue), S. sanguinis/universal biomass (red) and S. mutans/S. sanguinis (yellow). (c) Representative images of multispecies biofilms after EPS staining. Green: bacteria; red: EPS. (d) The quantitative data of EPS/bacterial amount in multispecies biofilms. (e) The pH values of culture media of multispecies biofilms. Results are presented as . Different lowercase letters indicate a significant intergroup difference and are marked according to mean value. (S: sucrose; ACE: acesulfame-K; ASP: aspartame; SAC: saccharin; SUC: sucralose.).

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