Research Article
Analysis and Construction of a Competitive Endogenous RNA Regulatory Network of Baicalin-Induced Apoptosis in Human Osteosarcoma Cells
Table 1
Primer sequences used for qRT-PCR analysis.
| | Gene name | | Primer sequence (5-3) |
| | lncRNA | | | | ENST00000607286 | Forward | GGTTCAGCTCGTGGAAGACA | | Reverse | TTTAAACGCGCCTACAGGGT |
| | ENST00000449500 | Forward | GATGGAACCCTGACCCTGTG | | Reverse | GACACACATAGACCGCGTGA |
| | GAPDH | Forward | CAGCCTCAAGATCATCAGCA | | Reverse | ACAGTCTTCTGGGTGGCAGT |
| | miRNA | | | | miR-486-3p | Forward | AACAAGTCGGGGCAGCTCA | | Reverse | GTCGTATCCAGTGCAGGGTCC | | RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACATCCTGT |
| | miR-1908-3p | Forward | ACCGGCCGCCGGCTCC | | Reverse | AGTGCAGGGTCCGAGGTATT | | RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCGGGGC |
| | miR-625-5p | Forward | AACCGGAGGGGGAAAGTTC | | Reverse | GTCGTATCCAGTGCAGGGT | | RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGGACTA |
| | U6 | Forward | CTCGCTTCGGCAGCACA | | Reverse | AACGCTTCACGAATTTGCGT |
| | mRNA | | | | TREX2 | Forward | CACCTGATCTCCAGTGACGG | | Reverse | TGGGGCCAGTTACACAAAGG |
| | B3GNT1 | Forward | TGCTCCCGGACAAGATATGAG | | Reverse | TGCCATCATCAGGATACCCA |
| | SLC9A7 | Forward | TGACTGGTGTTGTGACTGCT | | Reverse | AACGTGCTCCAGGACATGAG |
| | ZNF704 | Forward | TCTTCAGCAAAGCTCCCTGG | | Reverse | AACGTGCTCCAGGACATGAG |
| | STC2 | Forward | TGTAGTAGTTGAGCGCAGGC | | Reverse | AAGGAGTCGAGCAGGTGTTG |
| | WWC3 | Forward | CCCCGAGAAATTTCAGCCCT | | Reverse | CGTGCCACTCCGAACAAAAG |
| | WDR35 | Forward | ATGGAGACATTTGGTGCAACG | | Reverse | GAGGCTGCTATCACATGGGT |
| | DYRK2 | Forward | GTTCGTCAGCTTCAGGCTTC | | Reverse | CTTACTGCCGCCAATCGTGT |
| | KLF13 | Forward | ACGGGCGAGAAGAAGTTCAG | | Reverse | GCATTCCCGGGTGGAAGTTG |
| | KDM4B | Forward | ACCATCACTGTTGCTGGAGG | | Reverse | CACTTCTGGATGGCGAGGTT | | HELZ2 | Forward | CATCGCAGGTCCCCATCTAC | | Reverse | ATTTGGACCCAGAAGAGCCG |
| | MAN1A1 | Forward | AGCCCAGCCTAGGAAAGAGG | | Reverse | GGGAGACTCGTCAACTTCGC |
| | CPD | Forward | AGATTGTCTAAAGCATGGCAGT | | Reverse | TTCACACTTCCTGTAGCAGTT |
| | DPY19L1 | Forward | CTGGACCACGCTCCTGTTAG | | Reverse | TGCGAAAAGCCATCTCCCTT |
| | PPP1R3G | Forward | ATCATTGTGTCAGGCAGGGG | | Reverse | AAGCCAATTCAAACGGGTGC |
| | CAMSAP3 | Forward | CTTTTCTGGGTGGACACGAC | | Reverse | TTGCGGTATCGGATCGAGG |
| | ZSWIM5 | Forward | TTCCCCAGAGTGCCATTCAC | | Reverse | GCCAATGTAATTCACGCCCC |
| | ANO4 | Forward | ATCACTTTGCTGGCCTCCTC | | Reverse | TTCCTCAGTGCCTTGGTGTC |
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