[Retracted] Therapeutical Significance of Serpina3n Subsequent Cerebral Ischemia via Cytotoxic Granzyme B Inactivation

Figure 3

Expression of granzyme B was confirmed through (a) RFP by 293 T cells. (b) Immunoblot of cell lysates probed with granzyme B ~37 kDa. (c) Reprobed with 6-histidine reporter peptide representing same band weight ~37 kDa. (d) Fluorometric assay of granzyme B indicating significant inhibitory effect of Serpina3n in vitro at different concentrations. The data is expressed as and analyzed for statistical significance using two-way ANOVA with Bonferroni post hoc test taking as significant value (, , , and vs. positive control).

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