Application of Porcine Kidney-Derived Extracellular Matrix as Coating, Hydrogel, and Scaffold Material for Renal Proximal Tubular Epithelial Cell

Figure 3

Effects of renal ECM as a hydrogel material on human RPTE cell morphology, proliferation rate, and gene expression. Cells were cultured in the renal ECM hydrogel (1 mg) for 7 days. Hyaluronic acid (1 mg) was used as a control. (a) Cell morphology in the hydrogel, (b) cell proliferation rate and (c) gene expression for CD133, CD24, Pax2, pan-Cytokeratin, Megalin, Muc-1, AQP1, SGLT2, E-cadherin, β-catenin, and Vimentin. ECM: porcine kidney-derived extracellular matrix; HA: hyaluronic acid. Original magnification, 100x; scale bar, 100 μm. Different letters above bars indicate significant differences ().

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