FOXM1 Promotes Drug Resistance in Cervical Cancer Cells by Regulating ABCC5 Gene Transcription

Figure 4

FOXM1 and ABCC5 transporter expression is significantly increased in the Caski/Taxol cells compared with the Caski cells. (a) Protein and (b) mRNA expression levels of FOXM1 and ABCC5 were consistently significantly increased in the Caski/Taxol cells compared with the Caski cells. and vs. Caski cells. (c, d) FOXM1 knockdown significantly lowered the protein expression levels of FOXM1 and ABCC5, indicating a positive correlation between FOXM1 and ABCC5. vs. empty vector group. (e) ChIP analysis showed that the binding of FOXM1 to the FHK consensus motif of the abcc5 gene promoter was weak in the Caski cells, whereas in the paclitaxel-resistant Caski/Taxol cells, this binding was much stronger. ChIP: chromatin immunoprecipitation; Caski/Taxol: paclitaxel-resistant Caski cells; FOXM1: forkhead box M1; ABCC5: ATP-binding cassette subfamily C member 5.

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