Combination of Goniothalamin and Sol-Gel-Derived Bioactive Glass 45S5 Enhances Growth Inhibitory Activity via Apoptosis Induction and Cell Cycle Arrest in Breast Cancer Cells MCF-7

Figure 7

(a) Confluent monolayer of MCF-7 cells was mechanically wounded (scratch assay), leaving two wound edges separated by a 700-800 μm wide void (gap). The proliferation of untreated MCF-7 cells and the cells treated with BG, GTN, GTN-BG, and DOX on the void was monitored using the IncuCyte ZOOM® system at 10x magnification. The time-lapse images were taken at 0, 6, 12, and 21 hours, which are represented as A, B, C, and D, respectively. (b) The real-time proliferation of MCF-7 cells on the gap and the percentage of wound confluence were measured by using the IncuCyte™ ZOOM Live Cell System (Essen BioScience, USA) for up to 12 hours.

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