Research Article
Effects of Hypoxic Environment on Periodontal Tissue through the ROS/TXNIP/NLRP3 Inflammasome Pathway
Figure 3
Hypoxic environment induced by CoCl2 activates the ROS/TXNIP/NLRP3 inflammasome pathway in periodontal membrane fibroblasts. (a) Effects of different concentrations of CoCl2 on the proliferation of periodontal membrane fibroblasts cultured for 24 h and 48 h. (b, c) Immunofluorescence staining (IF) showed that the expressions of ROS, TXNIP, NLRP3, ASC, and caspase-1 were in the cells treated with different concentrations of CoCl2.. . (d) RT-PCR analysis of TXNIP, NLRP3, caspase-1, ASC, and IL-1β in HPDLCs cells treated with 200 μM and 400 μM CoCl2 for 24 h. (e) Western blotting analysis of TXNIP, NLRP3, caspase-1, ASC, and IL-1β in HPDLC cells treated with 200 μM and 400 μM for 24 h. The above data are presented as the , .
(a) |
(b) |
(c) |
(d) |
(e) |