Research Article
Effects of Hypoxic Environment on Periodontal Tissue through the ROS/TXNIP/NLRP3 Inflammasome Pathway
Figure 4
ROS mediates the inflammatory response of periodontal membrane fibroblasts under hypoxia. (a) ROS in HPDLCs after 24 h treatment with or without 2 mM NAC in the normal group and CoCl2-induced hypoxia environment. (b) Degree of aggregation of immunofluorescence staining (IF) in periodontal membrane fibroblasts after 24 h treatment with or without 2 mM NAC in the normal group and CoCl2-induced hypoxia environment. (c) RT-PCR analysis of TXNIP, NLRP3, ASC, caspase-1, and IL-1β in periodontal membrane fibroblasts after 24 h treatment with or without 2 mM NAC in the normal group and CoCl2-induced hypoxia environment. (d) Western blotting analysis of TXNIP, NLRP3, ASC, caspase-1, and IL-1β in periodontal fibroblasts treated with or without 2 mM NAC for 24 h in the normal group and CoCl2-induced hypoxia environment. The above data are presented as the , .
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