Circular RNA circ_0026218 Suppressed Atherosclerosis Progression via miR-338-3p/SIRT6 Axis

Figure 3

miR-338-3p is a target of circ_0026218. (a) The Circular RNA Interactome database was adopted to analyze the predicted binding sequence between miR-338-3p and circ_0026218. (b) This predicted binding was testified by dual-luciferase reporter gene assay. (c) The enrichment of circ_0026218 and miR-338-3p in HUVECs of anti-Ago2 group or the control group was exposed by RIP assay. (d) qRT-PCR was executed to probe miR-338-3p expressions in HUVECs with discrepant concentrations (0 μg/mL, 50 μg/mL, 75 μg/mL, or 100 μg/mL) of ox-LDL. (e) miR-338-3p expression in HUVECs treated with 100 μg/mL ox-LDL for 0 h, 24 h, 48 h, and 72 h was probed by qRT-PCR. (f) qRT-PCR exposed miR-338-3p expressions in HUVECs after circ_0026218 overexpression. , .

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