Circular RNA circ_0026218 Suppressed Atherosclerosis Progression via miR-338-3p/SIRT6 Axis

Figure 5

circ_0026218 inhibits ox-LDL-stimulated damage in HUVECs via targeting miR-338-3p. HUVECs were transfected with the circ_0026218 overexpression plasmid or/and miR-338-3p mimic, and treated with 100 μg/mL ox-LDL for 24 h. HUVECs without any treatment served as controls. (a) miR-338-3p expressions were probed by qRT-PCR. (b, c) SIRT6 mRNA and protein expressions were exposed by qRT-PCR and Western blot. (d) The viability of HUVECs after transfection was probed by CCK-8 method. (e–g) IL-1β, IL-6, and TNF-α contents were delved by ELISA. (h–j) The corresponding kits exposed the levels of ROS, MDA, and SOD in HUVECs after transfection. (k–l) The apoptosis was probed by flow cytometry. , , .

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