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Enzymes in Biorefinery: From Genes to Application

Call for Papers

Biorefinery applied to better management of plant biomass residues is considered a motivating alternative for the fuel industry, encompassing renewable sources that are not involved in greenhouse emission. By using different carbohydrate-active enzymes (CAZymes) from different organisms, it is possible to degrade cellulose and hemicellulose into fermentable sugars to produce biofuels and to convert lignin into relevant compounds, including polymers, aroma compounds, and pharmaceuticals.

For these purposes, several approaches can be applied to overcome bottlenecks and drawbacks that still limit enzyme utilization: omics approaches applied to gene prospection for the selection of highly active and stable enzymes, protein engineering for the obtainment of enzymes with improved characteristics, strain engineering to improve enzyme production, optimization of fermentation parameters for enzyme production, and optimization of enzyme use to obtain the desired product.

We would like to invite authors to contribute high-quality original research, as well as review articles, covering different aspects of enzymes used in biorefinery applied to plant biomass residues.

Potential topics include but are not limited to the following:

  • Omics techniques focusing on gene prospection
    • Genome sequencing of different organisms
    • Metagenome and metatranscriptome approaches
    • Proteomics approaches
  • Enzyme engineering for improvement of enzyme properties
    • Directed evolution of proteins
    • Rational design of proteins
  • Strain engineering
    • Gene deletion and overexpression to better produce specific enzymes or enzymatic cocktails
    • Cloning and expression of homologous and heterologous enzymes
  • Optimization of fermentation parameters
    • Use of response surface methodology to improve enzyme production
    • Evaluation of different cultivation media to reduce enzyme production costs
  • Optimization of enzyme application
    • Immobilization of enzymes to improve processes
    • Use of response surface technology to improve enzyme application

Authors can submit their manuscripts through the Manuscript Tracking System at

Submission DeadlineFriday, 13 April 2018
Publication DateAugust 2018

Papers are published upon acceptance, regardless of the Special Issue publication date.

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