Peptide Selank Enhances the Effect of Diazepam in Reducing Anxiety in Unpredictable Chronic Mild Stress Conditions in Rats

Table 3

Indicators of the behavior of rats that were exposed to unpredictable chronic stress at the EPM test before and after exposure to stress and the administration of a course of the test substances. 1, time spent in the open arms of the maze, s; 2, time spent in the closed arms of the maze, s; 3, number of hanging events from the open arms; 4, number of squares crossed; 5, number of rears.

Test substance		Saline	Selank	DZ	Selank + DZ
Behavioral measures		Ме; (25%–75%)	Ме; (25%–75%)	Ме; (25%–75%)	Ме; (25%–75%)
Measures of anxiety
1	Before	62,4	64,7	56,7	72,6
		(48,7–125,1)	(41,7–79,4)	(48,5–137,8)	(56,7–84,1)
	After	4,6	15,5	26,9	40,8^#
		(0–17,8)	(7–26,6)	(8,5–47,7)	(25,3–57,5)
2	Before	78,2	104,7	99,1	72,9
		(42,3–113,3)	(57,3–120,6)	(34,2–108,1)	(65,5–99,2)
	After	164,3	139,9	130,3	83^#
		(150,1–175,5)	(133,2–162,7)	(90,4–169)	(63,4–135,8)
3	Before	8,5	9	10,5	8
		(4–12)	(3–13)	(5–13)	(3–16)
	After	1,5	4,5	6	9
		(0–4)	(3–6)	(2–9)	(8–10)
Measures of locomotor activity
4	Before	23	39	25,5	40,5
		(23–47)	(33–59)	(14–36)	(23–49)
	After	27	22	16,5	25
		(21–34)	(15–30)	(12–26)	(22–38)
5	Before	8,5	6,5	7	7
		(6–12)	(5–10)	(5–12)	(6–12)
	After	5	5,5	7	5
		(4–8)	(3–8)	(5–10)	(3–7)

Note. Me: median; , comparison of indicators before and after exposure to the stress and the administration of a course of the test substances (Wilcoxon’s test); ^#, comparison of indicators in the subgroup of animals with related indicators in the control group of animals (Mann–Whitney test).