Research Article
Histopathological and Biochemical Assessment of Neuroprotective Effects of Sodium Valproate and Lutein on the Pilocarpine Albino Rat Model of Epilepsy
Figure 7
Photomicrographs of the hippocampus of albino rat group II (positive control group) show CA1, CA3, and DG. The image shows the (a) CA1 and (b) CA3 areas of hippocampus proper and (c) dentate gyrus. CA areas are formed of three layers: molecular layer (M), pyramidal (P), and polymorphic (P1) layers. In CA2 and CA3 areas, shrunken pyramidal cells with shrunken deeply stained pyknotic nuclei and deeply stained basophilic cytoplasm (↑) with perineuronal spaces within the pyramidal layer (red arrow) are seen, in CA1 and CA3 area pyramidal cells with karyolitic pale stained nuclei (yellow arrow). Dentate gyrus is formed of the molecular (M) layer, granule cell layer (G), and polymorphic layers (P2). An apparent decrease thickness of the granule cell layer is noticed compared to the control group. Shrunken granule cells with shrunken deeply stained pyknotic nuclei and deeply stained basophilic cytoplasm (↑) are seen. Halos are noticed around degenerated granule cells (red arrow). Some granule cells are seen with karyolitic nuclei (yellow arrow). Notice the pale vacuolated areas () in the molecular layer (M). Inset: higher magnification of the pyramidal cells and granular cells. Scale bar: 50 μm, positive control group II, H&E ×20 and inset ×40.
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