Figure 2: Downregulation of the mouse Pcyt2 gene by 25-hydroxycholesterol and the LXR-specific agonist TO901317. (a) C3H10T1/2 cells were cultured in the presence of 25-OH (10 ng/μL) or TO901317 (20 nM–20 μM) for 48 hours. Shown are promoter-luciferase reporter activities from four independent experiments performed in duplicates. The numerical values represent means ±S.D., with significant differences indicated as (*) at P < .05, (**) at P < .01 and (***) at P < .001. (b) Pcyt2 mRNAs (α and β) determined in 25-OH (left panel) and 1 μM TO901317 (right panel) treated cells. (c): Western blot showing that 25-OH (left panel) and 1 μM TO901317 (right panel) treatments of C3H10T1/2 cells reduced total ( 𝛼 + 𝛽 ) and α Pcyt2 proteins.