Identification and Partial Characterization of an L-Tyrosine Aminotransferase (TAT) from Arabidopsis thaliana

Figure 3

Recombinant expression and purification of At5g36160 from the E. coli strain BL21 Codon Plus RIPL. The enzyme was purified as described in Section 2. Along with the molecular mass markers (kDa) (lane 1), the gel shows the profile of 10 𝜇g soluble proteins extracted from uninduced (lane 2) and induced cells (lane 3). Lane 4 shows the profile of 1.0 𝜇g of the purified recombinant enzyme. The proteins were resolved on an SDS-PAGE gel containing 10% (w/v) acrylamide, and the gel was stained using Coomassie Blue.