Research Article

Purification and Characterization of a Mitogenic Lectin from Cephalosporium, a Pathogenic Fungus Causing Mycotic Keratitis

Table 2

Hapten inhibition studies with purified CSL.

GlycoproteinMinimum concentration required for inhibition, MIC ( g/50  l )

Mucin (from porcine stomach)0.785
Fetuin6.25
Asialofetuin12.5

D-(+)-Galactose, D-(+)-Glucose, D-(+)-Mannose, D-(+)-Arabinose, D-(+)-Fucose, L-(-)-Fucose, -D(+)-Glucose, 2-Deoxy-D-glucose, -L-Rhamnose, 1-Amino-1-deoxy- -D-glucose, N-Acetyl-D-galactosamine, N-Acetyl-D-glucosamine, N-Acetyl- -D-mannosamine, Methyl- -D-mannopyranoside, Methyl- -D-galactopyranoside, Methyl- -D-galactopyranoside, Methyl- -D-glucopyranoside, 4-Aminophenyl -D-galactopyranoside, 4-Aminophenyl -D-glucopyranoside, N-Acetyl neuraminic acid, -D-Galactose(1-4) -D-glucose ( -Lactulose), -D-Glucose(1-4)-D-glucose (cellobiose), 6- -D-Galactopyranosyl-D-glucopyranose (melibiose), and O- -D-Galactopyranosyl-(1-6)- -D-glucopyranosyl- -D-fructofuranoside (raffinose) did not inhibit the lectin activity up to concentrations of 200 mM. Also, ovalbumin (2 mg/ml), and the plant polysaccharides guar gum (1 mg/ml), gum acacia (10 mg/ml), were not inhibitory.