Research Article
Purification and Characterization of a Mitogenic Lectin from Cephalosporium, a Pathogenic Fungus Causing Mycotic Keratitis
Table 2
Hapten inhibition studies with purified CSL.
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D-(+)-Galactose, D-(+)-Glucose, D-(+)-Mannose, D-(+)-Arabinose, D-(+)-Fucose, L-(-)-Fucose, -D(+)-Glucose, 2-Deoxy-D-glucose, -L-Rhamnose, 1-Amino-1-deoxy--D-glucose, N-Acetyl-D-galactosamine, N-Acetyl-D-glucosamine, N-Acetyl--D-mannosamine, Methyl--D-mannopyranoside, Methyl--D-galactopyranoside, Methyl--D-galactopyranoside, Methyl--D-glucopyranoside, 4-Aminophenyl -D-galactopyranoside, 4-Aminophenyl -D-glucopyranoside, N-Acetyl neuraminic acid, -D-Galactose(1-4) -D-glucose (-Lactulose), -D-Glucose(1-4)-D-glucose (cellobiose), 6--D-Galactopyranosyl-D-glucopyranose (melibiose), and O--D-Galactopyranosyl-(1-6)--D-glucopyranosyl--D-fructofuranoside (raffinose) did not inhibit the lectin activity up to concentrations of 200 mM. Also, ovalbumin (2 mg/ml), and the plant polysaccharides guar gum (1 mg/ml), gum acacia (10 mg/ml), were not inhibitory. |