Bioseparation of Four Proteins from Euphorbia characias Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase

Table 1

Purification of Euphorbia latex peroxidase.

Step	Total protein    (mg)	Total activity   (nkat)	Specific activity    (nkat/mg)	Yield   (%)	Purification    (fold)
Acetone powder crude extract	3500	71545	20.4	100	1
Ammonium sulfate fractionation	534	40910	76.6	57	3.7
DEAE-cellulose chromatography	20.3	33414	1646	46.7	80.7
Hydroxylapatite column chromatography	5.2	28455	5500	39.8	270