Review Article

Bioseparation of Four Proteins from Euphorbia characias Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase

Table 2

Purification of Euphorbia latex nucleotide pyrophosphatase/phosphodiesterase.

StepTotal protein    (mg)Total activity    (nkat)Specific activity    (nkat/mg)Yield  (%)Purification    (fold)

Acetone powder crude extract350095002.71001
Controlled heating (60°C)360666518.5706.8
Ammonium sulfate fractionation210561526.75910
DEAE-cellulose chromatography53378071.34026