Bioseparation of Four Proteins from Euphorbia characias Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase

Table 4

Purification of Euphorbia latex copper amine oxidase.

Step	Total protein (mg)	Total activity (nkat)	Specific activity (nkat/mg)	Yield (%)	Purification (fold)
Acetone powder crude extract	3500	37917	10.8	100	1
Ammonium sulfate fractionation	534	33817	63	89.2	5.8
DEAE-cellulose chromatography	200.3	30000	150	79	13.8
Hydroxylapatite column chromatography	80.2	28067	350	74	32
𝜔-Aminohexy-Sepharose 4B column chromatography	41	23233	567	61	52.5