Mechanism(s) of Toxic Action of Zn²⁺ and Selenite: A Study on AS-30D Hepatoma Cells and Isolated Mitochondria

Figure 2

Induction of apoptosis in AS-30D cells by different concentrations of Zn²⁺ and selenite. The cells were cultivated in RPMI 1640 medium without (control) or with the indicated concentration (in μM) of Zn²⁺ and selenite for 24 h, stained with propidium iodide, and analysed by flow cytometry. Percentage of the sub-G₁ fraction, characteristic for apoptotic cells, is indicated in the upper left corner of each panel. A typical experiment out of at least three independent ones for each compound is shown.