Mechanism(s) of Toxic Action of Zn2+ and Selenite: A Study on AS-30D Hepatoma Cells and Isolated Mitochondria
Figure 3
Action of different concentrations of Zn2+ and selenite on the mitochondrial transmembrane potential after 24 h incubation with AS-30D cells. JC-1 is a cell-penetrating dye that accumulates within mitochondria maintaining high ΔΨmito and changes its emission fluorescence from green to red that can be followed by flow cytometry. The percentage of cells with red (R2) and green (R3) JC-1 fluorescence, reflecting high and low ΔΨmito, respectively, was estimated; R2 is indicated in the upper left corner of each panel. The protonophoric uncoupler CCCP plus the potassium ionophore valinomycin, acting together to completely collapse ΔΨmito, have always been used as the “positive” control. A typical experiment out of at least three independent ones is shown.
