Apple Procyanidins Suppress Amyloid -Protein Aggregation
Procyanidins dissolve Aβ aggregates in vitro. (a) Dose-dependent dissociation of Aβ42 aggregates by polyphenols determined using Th-T analysis. Samples were preincubated with Aβ42 for 48 hours (final concentration 20 μM) and then incubated with various concentrations of AP, PC, or MN for an additional 30 minutes. (b) Time-dependent Aβ42 disaggregation in the presence of polyphenols determined by the Th-T assay. Samples were preincubated with Aβ42 (20 μM) for 48 hours and then incubated with AP (360 μg/mL), PC (240 μg/mL), or MN (360 μg/mL). (Compared to vehicle groups, * (Dunnett's test)). Values are the means ± s.e.m., n = 3.