Figure 4: Fluorescence of AC-Hsp27 titrated with F-actin. Percent fluorescence enhancement at 485 nm as a function of actin concentration in F-buffer at 37°C. AC-Hsp27 = 1  M. The best fit of the data yielded an apparent dissociation constant M with the actin/Hsp27 molar stoichiometry fixed at 1.0. The error in is calculated from the root mean square deviation of data points from the fit.