Inhibition of the Mitochondrial Permeability Transition for Cytoprotection: Direct versus Indirect Mechanisms
Figure 2
MitoQ10 stimulates MPT in isolated cardiac mitochondria. (a) MitoQ10 increases the mitochondrial swelling induced by an oxidant stress. Mitochondria (25 μg of proteins) have been pretreated by the indicated doses of MitoQ10 and treated by 50 μM t- BHP + 10 μM . Absorbance at 540 nm has been registered for 60 min at 37°C. (b) MitoQ10 increases the mitochondrial depolarization induced by an oxidant stress. Mitochondria (25 μg of proteins) have been loaded with 2 μM Rhodamine 123, pretreated by the indicated doses of MitoQ10, and treated by 50 μM t-BHP + 10 μM . Fluorescence has been registered for 60 min at 37°C.
