Caveolin 3, Flotillin 1 and Influenza Virus Hemagglutinin Reside in Distinct Domains on the Sarcolemma of Skeletal Myofibers
Figure 1
Flotillin 1 resides in the DGC-deficient regions in structures near transverse tubule openings. A confocal section at the sarcolemma level indicates that flotillin 1 (a) appears as spots of irregular shape. Double staining for β-dystroglycan (b) and the merged image (c) show that dystroglycan and flotillin 1 do not colocalize. A confocal section at the sarcolemma level indicated that flotillin 1 (d) and DHPR (e) that mark transverse tubules are flanking each other, demonstrated in the merged image (f). A longitudinal confocal section in the middle of a myofiber indicates that flotillin 1 (g) exhibits intense staining that seems to be an extension of the DHPR staining (h) as demonstrated in the merged image (i). Immunoelectron microscopy for flotillin 1 in EDL section shows gold particles (arrowhead) in a structure beneath the sarcolemma near the transverse tubule neck (arrow) (j). Scale bars, 2 μm (a–i); 250 nm (j).
