Caveolin 3, Flotillin 1 and Influenza Virus Hemagglutinin Reside in Distinct Domains on the Sarcolemma of Skeletal Myofibers
Figure 5
Cholesterol depletion increases the water permeability of isolated myofibers. (a) Fluorescence micrographs of a calcein-loaded myofiber before (left) and 140 s after switching to the hypotonic buffer (right) that indicates decreased fluorescence. The myofiber was not treated with CDX. Scale bar, 50 μm. (b) Examples of the relative fluorescence (fluorescence at certain time point divided by the fluorescence at the beginning of the experiment) recordings for myofibers treated or not treated with CDX are shown. A sharp decline of the fluorescence intensity was often seen upon switching to the hypotonic buffer, owing to sample movement. (c) The change of fluorescence intensity is presented as a percentage between the value of fluorescence intensity in isotonic buffer just before and 140 s after switching from isotonic to hypotonic condition. (control) and 16 (CDX-treated) myofibers from five different isolations. ***.
