Impaired Caveolae Function and Upregulation of Alternative Endocytic Pathways Induced by Experimental Modulation of Intersectin-1s Expression in Mouse Lung Endothelium

Figure 4

Acute perturbation of ITSN-1s expression induces pleomorphic endocytic/transcytotic intermediates. (a–f) Representative EM images of membranous rings (a, a1, e, e.1, f), tubular elements (b, c, d, arrows) and enlarged endosomes (d, arrowheads), loaded with 8 nm Au-BSA and associated with caveolae-like morphology. Two tubular structures open to the lumen are shown in (c) and (e). The EM image shown in (e) was selected from a mouse lung specimen not perfused with 8 nm Au-BSA. Note also the severe dilation of the perivascular space (pvs) and the proteinaceous edema (a). Bars: 250 nm (a, b); 200 nm (c, d); 150 nm (f); 100 nm (a1, e).