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Biochemistry Research International
Volume 2012, Article ID 823597, 11 pages
Review Article

A Critical Appraisal of Quantitative Studies of Protein Degradation in the Framework of Cellular Proteostasis

1Departamento de Bioquímica, Instituto de Investigaciones Biomédicas “Alberto Sols” (UAM-CSIC), Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain
2Departamento de Matemáticas, Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain

Received 1 June 2012; Accepted 18 June 2012

Academic Editor: Dmitry Karpov

Copyright © 2012 Beatriz Alvarez-Castelao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Note that when the half-life of the protein is shorter than mRNA half-life, the protein decay by transcriptional shut-off experiments (red symbols curve) is governed by the mRNA decay (blue symbols) and gives an overestimate of protein half-life, bigger than the actual half-life of the protein determined by treatment of cells with protein synthesis inhibition(open symbols).

  1. Supplementary Material