Cardiomyopathy-Related Mutations in Cardiac Troponin C, L29Q and G159D, Have Divergent Effects on Rat Cardiac Myofiber Contractile Dynamics

Figure 7

Comparison of XB recruitment dynamic, 𝑏, in detergent-skinned fibers reconstituted with cTnC_WT,cTnC_L29Q, or cTnC_G159D. 𝑏 was estimated by fitting the R-D model to the force responses from muscle fibers to chirp-length perturbations [35]. One-way ANOVA was used to compare estimates of 𝑏 in cTnC_L29Q and cTnC_G159D fibers with the data from cTnC_WT fibers as controls. Values are expressed as mean ± SEM. Number of fibers tested in each group is as follows: cTnC_WT, 𝑛=15; cTnC_L29Q, 𝑛=10; cTnC_G159D, 𝑛=14. Minimal statistical significance was set at 𝛼=0.05. *𝑃<0.05; **𝑃<0.01