Review Article

Strategies to Identify Recognition Signals and Targets of SUMOylation

Figure 3

Sequence alignment SUMO consensus motifs. (a) Amino acid sequence alignment of the canonical SUMO consensus motif (Ψ represents a hydrophobic amino acid, K is the Lys modified by SUMO and x represents any amino acid). CM: canonical consensus motif. ICM: inverted consensus motif. PDSM: phosphorylation-dependent SUMO motif, NDSM: negatively charged amino-acid-dependent SUMO motif, HCSM: hydrophobic cluster SUMO motif. Amino acids in blue: basic, red: acid, green: hydrophobic, gray: phospho serine. (b) WebLogo [64] representation of the consensus motif of SUMOylated proteins reported in the phosphosite database on Fri Feb 03 08:31:18 EST 2012 (PhosphoSitePlus [65], http://www.phosphosite.org/). (c) The same SUMO motif aligned using Sequence Logo. Amino acid sequences are represented by frequency on the identified consensus.
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