Differential Expression of Matrix Metalloproteases in Human Fibroblasts with Different Origins
Figure 2
Proliferation, collagen expression, and basal MMP expression of cardiac, dermal, and pulmonary fibroblasts. (a) Growth curves were determined in complete growth medium for fibroblasts derived from heart, skin, and lung. Cardiac fibroblasts reveal a lower proliferation rate compared to dermal and pulmonary fibroblasts. (b) Collagen mRNA expression level was determined using TaqMan from fibroblasts incubated with or without 10 ng/mL TNF-α for 24 hours. Untreated dermal fibroblasts show a significantly higher expression level of collagen (§§§) compared to untreated cardiac and pulmonary fibroblasts, whereas only in dermal and pulmonary fibroblasts TNF-α treatment resulted in significantly decreased collagen expression compared to untreated controls (***). (c) Basal expression levels of different MMPs in dermal and pulmonary fibroblasts were compared to their expression in cardiac fibroblasts. The most obvious difference is shown for MMP1 and MMP3. All mRNA expression levels are shown as absolute expression using the formula .
