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Biochemistry Research International
Volume 2013 (2013), Article ID 436053, 8 pages
http://dx.doi.org/10.1155/2013/436053
Research Article

Collagen Content in Skin and Internal Organs of the Tight Skin Mouse: An Animal Model of Scleroderma

1Department of Microbiology and Immunology, Kimmel Cancer Center, Jefferson Medical College, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107-5541, USA
2Jefferson Institute of Molecular Medicine, Jefferson Medical College, Thomas Jefferson University, Suite 509 Bluemle Life Sciences Building, 233 South 10th Street, Philadelphia, PA 19107-5541, USA

Received 31 July 2013; Accepted 18 September 2013

Academic Editor: R. J. Linhardt

Copyright © 2013 Jayanthi Manne et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The Tight Skin mouse is a genetically induced animal model of tissue fibrosis caused by a large in-frame mutation in the gene encoding fibrillin-1 (Fbn-1). We examined the influence of gender on the collagen content of tissues in C57BL/6J wild type (+/+) and mutant Tight Skin (Tsk/+) mice employing hydroxyproline assays. Tissue sections were stained with Masson’s trichrome to identify collagen in situ. Adult Tsk/+ mice skin contains ~15% more collagen, on average, than skin from +/+ mice of the same gender. The heart of Tsk/+ males had significantly more collagen than that of +/+ males. No significant gender differences were found in lungs and kidney collagen content. Overall, the collagen content of Tsk/+ males and +/+ males was higher than that of their Tsk/+ and +/+ female counterparts, respectively. Our data confirm increased deposition of collagen in skin and hearts of Tsk/+ mice; however, the effects of the Tsk mutation on collagen content are both tissue specific and gender specific. These results indicate that comparative studies of collagen content between normal and Tsk/+ mice skin and internal organs must take into account gender differences caused by expression of the androgen receptor.