Collagen Content in Skin and Internal Organs of the Tight Skin Mouse: An Animal Model of Scleroderma

Table 1

Hydroxyproline content in skin of B6 +/+ and B6 Tsk/+ mice.

Group	Sex	Age in weeks	Number of mice	Site 1; Mean ± S.E	Site 2; Mean ± S.E	Site 3; Mean ± S.E
B6 +/+	F	24–27	6	45.7±1.6	57.0±2.1	61.1±1.6
B6 Tsk/+	F	23–25	6	51.1±2.5	68.0±2.5	73.9±3.7
				𝑃=0.50	𝑃=0.043	𝑃=0.12
B6 +/+	M	24–27	6	133.0±7.8	149.4±5.3	155.4±5.1
B6 Tsk/+	M	24–27	6	138.3±5.4	167.8±4.8	174.3±7.4
				𝑃=0.58	𝑃=0.032	𝑃=0.076

Hydroxyproline content is expressed as μg/4 mm skin punch. F: females; M: males. Site 1: proximal; Site 2: middle; Site 3: distal dorsum. S.E: standard error. Statistical comparisons were performed using the 𝑡-test following analysis of variance (see Section 2).