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Biochemistry Research International
Volume 2013 (2013), Article ID 731501, 18 pages
Review Article

Acetylcholinesterase Biosensors for Electrochemical Detection of Organophosphorus Compounds: A Review

1Department of Bio & Nanotechnology, Guru Jambeshwar University of Science & Technology, Hisar 125001, India
2Centre for Biotechnology, Maharshi Dayanand University, Rohtak 124001, India

Received 23 July 2013; Accepted 3 October 2013

Academic Editor: Seiji Shibasaki

Copyright © 2013 Vikas Dhull et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The exponentially growing population, with limited resources, has exerted an intense pressure on the agriculture sector. In order to achieve high productivity the use of pesticide has increased up to many folds. These pesticides contain organophosphorus (OP) toxic compounds which interfere with the proper functioning of enzyme acetylcholinesterase (AChE) and finally affect the central nervous system (CNS). So, there is a need for routine, continuous, on spot detection of OP compounds which are the main limitations associated with conventional analytical methods. AChE based enzymatic biosensors have been reported by researchers as the most promising tool for analysis of pesticide level to control toxicity and for environment conservation. The present review summarises AChE based biosensors by discussing their characteristic features in terms of fabrication, detection limit, linearity range, time of incubation, and storage stability. Use of nanoparticles in recently reported fabrication strategies has improved the efficiency of biosensors to a great extent making them more reliable and robust.