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Biochemistry Research International
Volume 2013 (2013), Article ID 764262, 6 pages
Research Article

Effects of Spirulina on Cyclophosphamide-Induced Ovarian Toxicity in Rats: Biochemical and Histomorphometric Evaluation of the Ovary

1Maltepe University School of Medicine, Department of Pathology, Maltepe, 34843 Istanbul, Turkey
2Maltepe University School of Medicine, Department of Urology, Maltepe, 34843 Istanbul, Turkey
3Maltepe University School of Medicine, Department of Obstetrics & Gynecology, Maltepe, 34843 Istanbul, Turkey
4Maltepe University School of Medicine, Department of Internal Medicine, Maltepe, 34843 Istanbul, Turkey
5Maltepe University School of Medicine, Department of General Surgery, Maltepe, 34843 Istanbul, Turkey
6Maltepe University School of Medicine, Department of Biochemistry, Maltepe, 34843 Istanbul, Turkey

Received 30 November 2012; Revised 22 March 2013; Accepted 10 April 2013

Academic Editor: Paul W. Doetsch

Copyright © 2013 Nese Arzu Yener et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Cyclophosphamide (Cyc) is known to cause ovotoxicity and infertility in women. Our aim is to investigate the possible ovotoxic effects of Cyc and possible antioxidant and protective effects of blue-green algae, Spirulina (Sp), in rat ovaries. Eighteen rats were given: group I ( , control); group II ( , CP), a single dose Cyc; group III ( , Sp+Cyc), 7 days Sp+single dose Cyc. Tissue malondialdehyde (MDA) levels, superoxide dismutase (SOD), and catalase (CAT) activities are assessed biochemically. Normal and atretic primordial and primary follicle counts for all sections obtained for each ovary are calculated. Mean number of follicle counts for each group are compared. In Sp+Cyc group, tissue MDA levels were significantly lower than those in the CP and higher than those in the C group ( + ). Tissue SOD activity was significantly higher in Sp+Cyc group than that in the CP group and lower than that in the C group ( + ). No statistically significant difference was found between the ovarian CAT activities in any group. Histomorphometrically, there was also no significant difference between the mean numbers of normal and atretic small follicle counts. Our results suggest that single dose Cyc has adverse effects on oxidant status of the ovaries and Sp has protective effects in Cyc-induced ovotoxicity.