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Biochemistry Research International
Volume 2014, Article ID 291458, 7 pages
http://dx.doi.org/10.1155/2014/291458
Research Article

Serum Prolidase Activity and Oxidative Stress in Diabetic Nephropathy and End Stage Renal Disease: A Correlative Study with Glucose and Creatinine

1Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India
2Department of Nephrology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India
3Division of Biostatistics, Department of Community Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India
4Department of Oral Pathology, Jaipur Dental College, Jaipur, Rajasthan 302020, India

Received 15 July 2014; Revised 21 August 2014; Accepted 21 August 2014; Published 8 September 2014

Academic Editor: Emanuel Strehler

Copyright © 2014 Akhilesh Kumar Verma et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Association of oxidative stress and serum prolidase activity (SPA) has been reported in many chronic diseases. The study was aimed at evaluating the correlation of glucose and creatinine to SPA and oxidative stress in patients with diabetic nephropathy (DN) and end stage renal disease (ESRD) concerned with T2DM. 50 healthy volunteers, 50 patients with T2DM, 86 patients with DN, and 43 patients with ESRD were considered as control-1, control-2, case-1, and case-2, respectively. Blood glucose, creatinine, SPA, total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI) were measured by colorimetric tests. SPA, TOS, and OSI were significantly increased in case-1 and case-2 than control-1 and control-2, while TAS was significantly decreased . Blood glucose was linearly correlated to SPA, TOS, TAS, and OSI in control-2, case-1 and case-2 . Serum creatinine was linearly correlated with SPA, TOS, TAS and OSI in control-2 and case-1 . In case-2, serum creatinine was significantly correlated with SPA only . Thus, the study concluded that SPA and oxidative stress significantly correlated with blood glucose and creatinine. SPA, TOS, TAS, and OSI can be used as biomarkers for diagnosis of kidney damage.