O Serogroup-Specific Touchdown-Multiplex Polymerase Chain Reaction for Detection and Identification of Vibrio cholerae O1, O139, and Non-O1/Non-O139

Table 1

Primer sequences^a used in touchdown-multiplex PCR.

Primer set	Sequence (5^′ to 3^′)	Tm (^°C)	Direction	Amplicon size (bp)	Reference
Universal primers^b
MVCO1	CAC CAA GAA GGT GAC TTT ATT GTG	68	Forward	588	Nandi et al., 2000 [30]
MVCO2	GAA CTT ATA ACC ACC CGC G	58	Reverse
O1-specific primers^c
MVCO3	GAC TGT CAG CTG GCG GAA	58	Forward	364	This study
MVCO4	GTT GGC GTA TTA CGG TAC	54	Reverse
O139-specific primers^d
MVCO5	GCG TTT ATC GCC GGT CGA C	62	Forward	256	This study
MVCO6	GTA ACT TGG TAC AAT CTC G	54	Reverse

All were originally derived from the nucleotide sequences (positions) available from the GenBank accession numbers ^bAE003853 (819612–820199), ^bCP000626 (404919–405506), ^cAE003852 (264415–264778), ^cCP000627 (2789549–2789912), and ^dU47057 (902–1157); from the EMBL accession numbers ^bX51948 (221–808), ^cX59554 (18246–18609), ^cY07788 (199–562), and ^dY07786 (11878–12133); or from the DDBJ accession number ^dAB012956 (32652–32907).