Table of Contents Author Guidelines Submit a Manuscript
Biochemistry Research International
Volume 2014 (2014), Article ID 348712, 7 pages
http://dx.doi.org/10.1155/2014/348712
Research Article

A Metabolic Study on Colon Cancer Using 1H Nuclear Magnetic Resonance Spectroscopy

1Biochemistry Department, Pasteur Institute of Iran, Tehran 1316943551, Iran
2Amir Alam Hospital, North Sa’adi Avenue, Tehran 8915964665, Iran
3Imam Khomeini Hospital, Dr. Gharib Road, Tehran 1419733141, Iran
4Biotechnology Department, Pasteur Institute of Iran, Tehran 1316943551, Iran
5Payame Noor University, Tehran 19569, Iran
6Sharif University, Azadi Avenue, Tehran 11559567, Iran

Received 11 May 2014; Accepted 13 July 2014; Published 14 August 2014

Academic Editor: R. J. Linhardt

Copyright © 2014 Zahra Zamani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Colorectal carcinoma is the third cause of cancer deaths in the world. For diagnosis, invasive methods like colonoscopy and sigmoidoscopy are used, and noninvasive screening tests are not very accurate. We decided to study the potential of 1HNMR spectroscopy with metabolomics and chemometrics as a preliminary noninvasive test. We obtained a distinguishing pattern of metabolites and metabolic pathways between colon cancer patient and normal. Methods. Sera were obtained from confirmed colon cancer patients and the same number of healthy controls. Samples were sent for 1HNMR spectroscopy and analysis was carried out Chenomex and MATLAB software. Metabolites were identified using Human Metabolic Data Base (HDMB) and the main metabolic cycles were identified using Metaboanalyst software. Results. 15 metabolites were identified such as pyridoxine, orotidine, and taurocholic acid. Main metabolic cycles involved were the bile acid biosynthesis, vitamin B6 metabolism, methane metabolism, and glutathione metabolism. Discussion. The main detected metabolic cycles were also reported earlier in different cancers. Our observations corroborated earlier studies that suggest the importance of lowering serum LCA/DCA and increasing vitamin B6 intake to help prevent colon cancer. This work can be looked upon as a preliminary step in using 1HNMR analysis as a screening test before invasive procedures.