A Nonhost Peptidase Inhibitor of ~14 kDa from Butea monosperma (Lam.) Taub. Seeds Affects Negatively the Growth and Developmental Physiology of Helicoverpa armigera
Figure 1
(a) Purification of BmPI using F2 dialyzed fraction on Sephadex G-75. Each fraction collected through the column is of 4.5 mL at an initial flow rate of 0.3 mL min−1. (b) Trypsin affinity purification of BmPI using fractions (42–58) from Sephadex G-75 and protein profile on 12% SDS-PAGE under reducing conditions. The retained BmPI was eluted with 100 mM HCl. Inset SDS-PAGE of pooled fraction (21 to 36): molecular weight markers are on the left and BmPI from trypsin affinity column chromatography are indicated on the right.
