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Biochemistry Research International
Volume 2014, Article ID 436848, 6 pages
http://dx.doi.org/10.1155/2014/436848
Research Article

Antiatherosclerotic and Cardioprotective Potential of Acacia senegal Seeds in Diet-Induced Atherosclerosis in Rabbits

1Department of Zoology, Jai Narain Vyas University, Jodhpur 342001, India
2Molecular Medicine and Toxicology Lab, School of Life Sciences, Devi Ahilya University, Indore, Madhya Pradesh 452001, India

Received 31 July 2014; Accepted 19 November 2014; Published 7 December 2014

Academic Editor: Gary A. Lorigan

Copyright © 2014 Heera Ram et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Acacia senegal L. (Fabaceae) seeds are essential ingredient of “Pachkutta,” a specific Rajasthani traditional food. The present study explored antiatherosclerotic and cardioprotective potential of Acacia senegal seed extract, if any, in hypercholesterolemic diet-induced atherosclerosis in rabbits. Atherosclerosis in rabbits was induced by feeding normal diet supplemented with oral administration of cholesterol (500 mg/kg body weight/day mixed with coconut oil) for 15 days. Circulating total cholesterol (TC), HDL-cholesterol (HDL-C), LDL-cholesterol (LDL-C), triglycerides, and VLDL-cholesterol (VLDL-C) levels; atherogenic index (AI); cardiac lipid peroxidation (LPO); planimetric studies of aortal wall; and histopathological studies of heart, aorta, kidney, and liver were performed. Apart from reduced atherosclerotic plaques in aorta () and increased lumen volume (), administration with ethanolic extract of Acacia senegal seeds (500 mg/kg/day, p.o.) for 45 days to atherosclerotic rabbits significantly lowered serum TC, LDL-C, triglyceride, and VLDL-C levels and atherogenic index as compared to control. Atherogenic diet-induced cardiac LPO and histopathological abnormalities in aorta wall, heart, kidney, and liver were reverted to normalcy by Acacia senegal seed extract administration. The findings of the present study reveal that Acacia senegal seed extract ameliorated diet-induced atherosclerosis and could be considered as lead in the development of novel therapeutics.

1. Introduction

Atherosclerosis is a chronic disease characterized by lipid deposition and inflammation in arterial wall [1]. Accumulation of oxidized cholesterol through a cascade of gradual developing processes results in an unstable atherosclerotic plaque that ultimately bursts and gives rise to myocardial infarction [1, 2]. Atherosclerosis is mainly influenced by hypercholesterolemia and dyslipidemia that are developed through various risk factors, that is, hereditary, sedentary lifestyle, diabetes, and high fat diet consumption [1, 3, 4]. A number of pharmacological agents are available in the market to manage dyslipidemia and atherosclerosis; however the drugs are reported to induce adverse drug reactions [57]. Interestingly, herbal nutritional supplement has a potential to ameliorate cardiovascular diseases at different steps in their development without any known side effect [5, 7, 8].

The resident population of Western Rajasthan consumes a specific kind of long-established food known as “Pachkutta” and its main components are Acacia senegal L. (Fabaceae) seeds, pod of Prosopis cineraria L. (Fabaceae), and Capparis decidua L. (Capparaceae) fruit. In general, Rajasthani people who incorporate “Pachkutta” and some long-established foods in their diet are found to report almost nil incidences of cardiovascular system related diseases. Acacia senegal, commonly known as Gum Arabica, Kumath, and Rfaudraksha, is a drought or arid region tree. Officinal parts of Acacia senegal such as seeds, fruits, leaves, gum, and bark are rich in polyphenols, flavonoids, tannins, saponins, and alkaloids [9, 10]. While Acacia senegal gum is used for soothing mucous membranes of the intestine and to treat inflamed skin [11], it is also reported to cure bleeding, bronchitis, malaria, diarrhea, gonorrhea, leprosy, typhoid fever, and upper respiratory tract infections besides possessing antiplatelet and antifertility activities [1214].

The present study was planned to investigate antiatherosclerotic and cardioprotective role of Acacia senegal seed extract, if any, using high fat diet-induced atherosclerotic rabbits as working model. Atherosclerotic plaques in aorta; lumen volume; cardiac lipid peroxidation (LPO); circulating total cholesterol (TC), HDL-cholesterol (HDL-C), LDL-cholesterol (LDL-C), triglycerides, and VLDL-cholesterol (VLDL-C) levels; atherogenic index (AI); and organ (heart, aorta, kidney, and liver) weight were considered as key parameters. Planimetric studies of aortal wall and histopathological studies of heart, aorta, kidney, and liver were studied as supporting parameters to correlate with altered conditions.

2. Materials and Methods

2.1. Chemicals and Plant Material

Standard drug statin (Atorvastatin) was purchased from a registered local medical store. Glacial acetic acid, hydrogen peroxide, diethylene triamine penta acetic acid, sodium dodecyl sulfate, ethylene diamine tetra acetic acid (EDTA), HCl, xylene, stain chemicals, and sulfuric acid were obtained from E. Merck Ltd., Mumbai, India. All other chemicals were of reagent grade and were purchased from Loba Chemie, Mumbai, India. Acacia senegal L. seeds were procured from a registered supplier M/S Bharat seed distributors, Jodhpur, Rajasthan, India, and authenticated by taxonomist. Seeds were ground to powder form and extracted in 70% ethanol for 18 h using soxhlet apparatus; 2% of ground powder was recovered in extract. A voucher specimen was deposited in departmental herbarium (HR-2012-AS).

2.2. Animals and Induction of Atherosclerosis

Colony bred adult New Zealand white male rabbits, weighing  kg, were used as working model. Animals were housed in metallic wire gauge cages, under controlled light (12 h light: dark) and temperature (°C) controlled room with the provision of standard laboratory feed (Hindustan Lever Ltd., Mumbai, India). Food was supplemented with green leafy and seasonal vegetables and water ad libitum. Thirty-two healthy rabbits were divided into four groups of eight each. Animals of group 1 receiving the vehicle, distilled water (5 mL/animal/day, p.o.) served as control, while those of groups 2, 3, and 4 received high fat diet (500 mg cholesterol mixed with 5 mL coconut oil/kg, p.o.) for 15 days to induce atherosclerosis [15]. The average consumption of diet was 200 g/rabbit/day. After 15 days animals of groups 1 and 2 received vehicle, distilled water (5 mL/animal/day, p.o.) and those of groups 3 and 4 received Acacia senegal seed extract (500 mg/kg/day, p.o.) and Atorvastatin (0.25 mg/kg/day, p.o.), respectively, for 45 days. Drug or vehicle administration was done by gastric intubation method. Institutional Animal Ethics Committee (IAEC) approved the experimental protocols.

2.3. Collection of Blood Samples and Planimetric Studies

After completion of 60 days, overnight fasting animals were sacrificed under ether anesthesia. Blood samples were collected by cardiac puncture method and kept in EDTA coated test tubes and normal tubes for biochemical and hematological assessments. Planimetric studies of aorta wall and atherosclerotic plaque were performed by using Camera Lucida and measured layers (intima, media, and adventitia), lumen volume, and atherosclerotic plaque area, as routinely done in our laboratory [15].

2.4. Biochemical Estimations in Tissue and Plasma

Plasma fasting glucose concentration was measured by following enzymatic (glucose oxidase/peroxidase) method, as described elsewhere [16, 17]. Cardiac LPO was studied in microsomal fraction by the reaction of thiobarbituric acid with malondialdehyde in acidic condition [6, 17]. While for the estimation of TC and HDL-C spectrophotometric methods of Allain et al. [18] and Finley and coworkers [19], respectively, and for triglycerides standard, protocol [20] was followed. LDL-C, VLDL-C, and atherogenic index (AI) were calculated using Friedewald’s formula [17, 21].

2.5. Histopathological Investigations

After exsanguinations heart, aorta, liver, and both the kidneys were removed, quickly freed from blood clots, and washed thoroughly with phosphate buffered saline (0.1 M, pH 7.4) and kept in Bouin’s fixatives. Fixed sections were stained with hematoxylin-eosin after passing through ethanol series and observed under Microscope to study hypercholesterolemic diet-induced alterations in histoarchitecture [15, 22].

2.6. Statistical Analyses

Values of biochemical assessment, organs weights, and planimetric studies were expressed as mean ± standard error of mean (S.E.M.) and analyzed for ANOVA and post hoc Dunnett’s -test using SPSS 17 trial version for windows.

3. Results

3.1. Plasma Lipid Profile and Cardiac LPO

Administration of atherosclerotic diet to rabbits for 15 days increased cardiac LPO, AI value, and circulating TC, LDL-C, VLDL-C, and triglyceride levels (Table 1; Figure 1). Acacia senegal seed extract administration for 45 days to atherosclerotic animals reduced TC, triglycerides, LDL-C, VLDL-C, and atherogenic index and cardiac LPO (Table 1; Figure 2).

tab1
Table 1: Effects of Acacia senegal seed extract (AS, 500 mg/kg/day, p.o.) or statin (0.25 mg/kg/day, p.o.) treatment for 45 days on the alteration in plasma concentration of total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and very low-density lipoprotein cholesterol (VLDL-C), all in mg/dL and on TC/HDL-C ratio and on atherogenic index in hypercholesterolemic (Chol) diet fed atherosclerotic male rabbits.
436848.fig.001
Figure 1: Effects of Acacia senegal seed extract (AS, 500 mg/kg/day, p.o.) or statin (0.25 mg/kg/day, p.o.) treatment for 45 days on the alteration in cardiac lipid peroxidation (nM MDA/mg protein/h) in hypercholesterolemic (Chol) diet fed atherosclerotic male rabbits. Data are means ± S.E.M. (); a, and c, as compared to the respective control values and g, as compared to the respective values of the Chol diet fed group.
436848.fig.002
Figure 2: Effects of Acacia senegal seed extract (AS, 500 mg/kg/day, p.o.) or statin (0.25 mg/kg/day, p.o.) treatment for 45 days on atherogenic index in hypercholesterolemic (Chol) diet fed atherosclerotic male rabbits. Data are means ± S.E.M. (); a, and c, as compared to the respective control values and g, as compared to the respective values of the Chol diet fed group.
3.2. Planimetric Studies, Histological Observations, and Organs Weight

In vehicle treated control group, aorta wall consisted of three layers, that is, intima, media, and adventitia as normal histoarchitecture. Administration of high fat diet along with cholesterol to rabbits caused a bulging structure of atheromatous plaque at intimal surface (Figure 3). Acacia senegal seed extract and statin administration to atherosclerotic rabbits reduced atheromatous plaques at different degrees and increased lumen volume (Table 2). Atheromatous plaque development and fat deposition caused abnormal histoarchitecture of aortal wall in hypercholesterolemic group (Figure 3). On one hand treatment with Acacia senegal extract reduced plaque area up to 82.18%, intima area 18.19%, and total wall 28.84% and on the other hand media area and lumen volume were increased to 40.85% and 41.39%, respectively (Table 2). Nonsignificant alterations in heart and kidney weight were observed after hypercholesterolemic diet feeding as well as Acacia senegal seed extract and statin administration, except aorta and liver (Table 3).

tab2
Table 2: Effects of Acacia senegal seed extract (AS, 500 mg/kg/day, p.o.) or statin (0.25 mg/kg/day, p.o.) treatment for 45 days on the alteration in total wall area (TWA), lumen, intima, media, adventitia, and plaque, all in mm (images of Camera Lucida at 4 × 8 magnification; all in % of total area) in hypercholesterolemic (Chol) diet fed atherosclerotic male rabbits.
tab3
Table 3: Effects of Acacia senegal seed extract (AS, 500 mg/kg/day, p.o.) or statin (0.25 mg/kg/day, p.o.) treatment for 45 days on the alteration in liver, heart, kidney, and aorta weight, all in gm/Kg body weight in hypercholesterolemic (Chol) diet fed atherosclerotic male rabbits.
fig3
Figure 3: Effects of Acacia senegal seed extract (AS, 500 mg/kg/day, p.o.) or statin (0.25 mg/kg/day, p.o.) treatment for 45 days on the alteration in aorta wall in hypercholesterolemic (Chol) diet fed male rabbits (200x H&E).

4. Discussion

Findings of the present study reveal that hypercholesterolemic diet fed rabbits not only decreased aorta and lumen area, but also developed dyslipidemia and atherosclerosis. Hypercholesterolmic diet-induced dyslipidemia is quite expected as the diet itself is composed of cholesterol and lipid. Dietary factors play an important role in the development of various diseases, including that of cardiovascular diseases. Landmark epidemiological studies reflect that diets rich in fruits, herbs, and spices are associated with a low risk of cardiovascular diseases [8, 23, 24]. Mammals are sensitive to atherosclerosis, induced by dietary cholesterol because they are unable to increase sterol excretion, resulting into enhanced liver export of cholesteryl ester-rich lipoproteins into the circulation [4, 25, 26].

In the present study, high fat diet-induced hypercholesterolemia and atherosclerosis are an outcome of accumulation of cholesterol by various mechanisms, as reported earlier [5, 27]. However, treatment with Acacia senegal seed extract reduced total cholesterol, LDL-cholesterol, and VLDL-cholesterol as well as AI, reflecting the potential of plant extract in amelioration of diet-induced atherosclerosis and cardiac toxicity. However, hypolipidemic and antiatherosclerotic activities of Acacia senegal seed extract might be an outcome of its active ingredients [9, 28]. Indeed, histoprotective nature of Acacia senegal seed extract was further supported by the observations made on the volumes of total wall area, lumen, intima, media, adventitia, and plaque.

In fact, plant derived active principles, namely, polyphenols, tannins, flavonoids, polyphenols, alkaloids, and so forth, are reported to reduce cholesterol production in liver and to inhibit the activities of cholesterol biosynthetic enzymes [23, 29]. This fact was further supported by histological examinations made on the tissues of Acacia senegal seed extract treated aorta, revealing restoration of aortal walls and volume to normalcy and absence of plaques. However, antiatherosclerotic and hypolipidemic effects might be an outcome of depletions of deposited fatty contents and reduced peroxidation of lipid contents following Acacia senegal seed extract, as suggested by earlier workers [5, 3032]. Interestingly, following Acacia senegal seed extract administration, aorta and liver weights were also brought to normal. Indeed, plant derived active principles such as polyphenols, saponins, alkaloids, and flavonoids are reported to suppress LDL-receptors and promote lipolytic activities [4, 8]. While restoration of tissue histology following plant extract administration might be an outcome of inhibitions in the activities of HMG CoA reductase enzyme which catalyzes conversion of HMG CoA to mevalonate, a rate limiting step in the formation of endogenous cholesterol leading to the decrease in the intracellular status of cholesterol [25, 33], it is speculated that Acacia senegal seed might lower LDL-cholesterol through inhibiting hepatic cholesterol biosynthesis and VLDL-cholesterol production which is the source of LDL-cholesterol production. This fact is further supported by the findings made on lipid profile, which was reduced following plant extract administration. These alterations also followed suppression of VLDL particle maturation and secretion from liver, decrease the VLDL level in plasma, and further decrease the LDL level and AI value [31].

Atherosclerotic diet fed animals also exhibited damage in cardiac tissues as evidenced by increase in LPO. The enhanced tissue LPO along with altered cardiac and aorta histology and dyslipidemia manifests the toxic nature of the hypercholesterolemic diet in different tissues, as suggested earlier by other workers [4, 27], while Acacia senegal extract administration resulted in amelioration of oxidative stress evidenced by the reduced LPO and restoration of cardiac histology to normalcy as well as lipid profiles. In fact, following plant extract treatment reversal of altered cardiac histopathology and LPO might be an outcome of its active principles, as plant extracts are reported to reduce oxidative stress [14, 3436].

In conclusion, the findings of the present study provide justification for inclusion of Acacia senegal seeds in traditional Rajasthani diet “Pachkutta” as the extract ameliorated hypercholesterolemic diet-induced atherosclerosis without any adverse effects and could be considered as lead in the development of novel therapeutics. However, studies on the isolated active principles are required to recommend the seeds as therapeutics for cardiovascular diseases.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

Acknowledgment

Financial support from the University Grants Commission, New Delhi, India, under Career Research Award no. F.30-11/2011(SA-II) dated January 16, 2012, to R. Jatwa is gratefully acknowledged.

References

  1. A. J. Lusis, “Atherosclerosis,” Nature, vol. 407, no. 6801, pp. 233–241, 2000. View at Publisher · View at Google Scholar · View at Scopus
  2. C. Weber and H. Noels, “Atherosclerosis: current pathogenesis and therapeutic options,” Nature Medicine, vol. 17, no. 11, pp. 1410–1422, 2011. View at Publisher · View at Google Scholar · View at Scopus
  3. K. J. Williams, J. E. Feig, and E. A. Fisher, “Rapid regression of atherosclerosis: insights from the clinical and experimental literature,” Nature Clinical Practice Cardiovascular Medicine, vol. 5, no. 2, pp. 91–102, 2008. View at Publisher · View at Google Scholar · View at Scopus
  4. M. J. Dessi, A. Noce, P. Bertucci et al., “Atherosclerosis, dyslipidemia and inflammation: the significant role of polyunsaturated fatty acids,” Inflammation, vol. 2013, Article ID 191823, 23 pages, 2013. View at Publisher · View at Google Scholar
  5. G. Valli and E.-G. V. Giardina, “Benefits, adverse effects and drug interactions of herbal therapies with cardiovascular effects,” Journal of the American College of Cardiology, vol. 39, no. 7, pp. 1083–1095, 2002. View at Publisher · View at Google Scholar · View at Scopus
  6. R. Jatwa and A. Kar, “Positive influence of Centchroman on cardiovascular system and tissue lipid peroxidation in rats,” Contraception, vol. 76, no. 5, pp. 408–412, 2007. View at Publisher · View at Google Scholar · View at Scopus
  7. G. Sreejith, M. Jayasree, P. G. Latha et al., “Hepatoprotective activity of Oxalis corniculata L. ethanolic extract against paracetamol induced hepatotoxicity in Wistar rats and its in vitro antioxidant effects,” Indian Journal of Experimental Biology, vol. 52, no. 2, pp. 147–152, 2014. View at Google Scholar · View at Scopus
  8. G. Lalitha, P. Poornima, A. Archanah, and V. V. Padma, “Protective effect of neferine against isoproterenol-induced cardiac toxicity,” Cardiovascular Toxicology, vol. 13, no. 2, pp. 168–179, 2013. View at Publisher · View at Google Scholar · View at Scopus
  9. A. A. Al-Yahya, A. A. Al-Majed, A. M. Gado et al., “Acacia senegal gum exudate offers protection against cyclophosphamide-induced urinary bladder cytotoxicity,” Oxidative Medicine and Cellular Longevity, vol. 2, no. 4, pp. 207–213, 2009. View at Publisher · View at Google Scholar · View at Scopus
  10. H. A. Almahy and O. D. Nasir, “Phytochemical and mineral content of the leaves of four Sudanese Acacia species,” Journal of Stored Products and Postharvest Research, vol. 2, no. 11, pp. 221–226, 2011. View at Google Scholar
  11. R. G. Marwah, M. O. Fatope, R. A. Mahrooqi, G. B. Varma, H. A. Abadi, and S. K. S. Al-Burtamani, “Antioxidant capacity of some edible and wound healing plants in Oman,” Food Chemistry, vol. 101, no. 2, pp. 465–470, 2007. View at Publisher · View at Google Scholar · View at Scopus
  12. A. El-Thar, G. M. H. Satti, and S. A. Khalid, “Antiplasmodial activity of selected Sudanese medicinal plants with emphasis on Acacia nilotica,” Phytotherapy Research, vol. 13, no. 6, pp. 474–521, 1999. View at Google Scholar
  13. M. L. Saini, R. Sainai, S. Roy, and A. Kumar, “Comparative pharmacognostical and antimicrobial studies of Acacia species (Mimoceae),” Journal of Medicinal Plants Research, vol. 2, no. 12, pp. 378–386, 2009. View at Google Scholar
  14. Y.-T. Tung, J.-H. Wu, C.-C. Huang et al., “Protective effect of Acacia confusa bark extract and its active compound gallic acid against carbon tetrachloride-induced chronic liver injury in rats,” Food and Chemical Toxicology, vol. 47, no. 6, pp. 1385–1392, 2009. View at Publisher · View at Google Scholar · View at Scopus
  15. A. Purohit and H. Ram, “Hypolipidemic and antiatherosclerotic effects of Prosopis cineraria bark extract in experimentally induced hyperlipidemic rabbits,” Asian Journal of Pharmaceutical and Clinical Research, vol. 5, no. 3, pp. 106–109, 2012. View at Google Scholar · View at Scopus
  16. P. Trinder, “Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen,” Journal of Clinical Pathology, vol. 22, no. 2, pp. 158–161, 1969. View at Publisher · View at Google Scholar · View at Scopus
  17. R. Jatwa and A. Kar, “Cardio-protective role of terazosin is possibly mediated through alteration in thyroid function,” European Journal of Pharmacology, vol. 551, no. 1–3, pp. 87–91, 2006. View at Publisher · View at Google Scholar · View at Scopus
  18. C. C. Allain, L. S. Poon, and C. S. G. Chan, “Enzymatic determination of total serum cholesterol,” Clinical Chemistry, vol. 20, no. 4, pp. 470–475, 1974. View at Google Scholar · View at Scopus
  19. P. R. Finley, R. B. Schifman, R. J. Williams, and D. A. Lichti, “Cholesterol in high-density lipoprotein: use of Mg2+/dextran sulfate in its enzymic measurement,” Clinical Chemistry, vol. 24, no. 6, pp. 931–933, 1978. View at Google Scholar · View at Scopus
  20. P. Fossati and L. Prencipe, “Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide,” Clinical Chemistry, vol. 28, no. 10, pp. 2077–2080, 1982. View at Google Scholar · View at Scopus
  21. N. Fukuyama, K. Homma, N. Wakana et al., “Validation of the Friedewald equation for evaluation of plasma LDL-cholesterol,” Journal of Clinical Biochemistry and Nutrition, vol. 43, no. 1, pp. 1–5, 2008. View at Publisher · View at Google Scholar · View at Scopus
  22. J. Bancroft and A. Stevens, Theory and Practice of Histological Techniques, Churchill Livingstone, New York, NY, USA, 1982.
  23. S. Kim, C.-K. Kim, K.-S. Lee et al., “Aqueous extract of unripe Rubus coreanus fruit attenuates atherosclerosis by improving blood lipid profile and inhibiting NF-κB activation via phase II gene expression,” Journal of Ethnopharmacology, vol. 146, no. 2, pp. 515–524, 2013. View at Publisher · View at Google Scholar · View at Scopus
  24. W. Qidwai and T. Ashfaq, “Role of garlic usage in cardiovascular disease prevention: an evidence-based approach,” Evidence-Based Complementary and Alternative Medicine, vol. 2013, Article ID 125649, 9 pages, 2013. View at Publisher · View at Google Scholar · View at Scopus
  25. K. Pahan, “Lipid-lowering drugs,” Cellular and Molecular Life Sciences, vol. 63, no. 10, pp. 1165–1178, 2006. View at Publisher · View at Google Scholar · View at Scopus
  26. W. Insull Jr., “The pathology of atherosclerosis: plaque development and plaque response to medical treatment,” The American Journal of Medicine, vol. 122, no. 1, pp. S3–S14, 2009. View at Publisher · View at Google Scholar · View at Scopus
  27. B. G. Madhumathi, M. V. Venkataranganna, S. Gopumadhavan, M. Rafiq, and S. K. Mitra, “Induction and evaluation of atherosclerosis in New Zealand white rabbits,” Indian Journal of Experimental Biology, vol. 44, no. 3, pp. 203–208, 2006. View at Google Scholar · View at Scopus
  28. A. Khatri, A. Rathore, and U. K. Patil, “Prosopis cineraria (L.) druce: a boon plant of desert—an overview,” International Journal of Biomedical and Advance Research, vol. 1, no. 5, pp. 141–149, 2010. View at Google Scholar
  29. Y. H. Cho, C. R. Ku, Z.-Y. Hong et al., “Therapeutic effects of water soluble Danshen extracts on atherosclerosis,” Evidence-Based Complementary and Alternative Medicine, vol. 2013, Article ID 623639, 11 pages, 2013. View at Publisher · View at Google Scholar · View at Scopus
  30. A. Purohit and K. B. Vyas, “Antiatherosclerotic effect of Caparis decidua fruit extract in cholesterol-fed rabbits,” Pharmaceutical Biology, vol. 44, no. 3, pp. 172–177, 2006. View at Publisher · View at Google Scholar · View at Scopus
  31. N. Khanna, D. Arora, S. Halder et al., “Comparative effect of Ocimum sanctum, Commiphora mukul, folic acid and ramipril on lipid peroxidation in experimentally-induced hyperlipidemia,” Indian Journal of Experimental Biology, vol. 48, no. 3, pp. 299–305, 2010. View at Google Scholar · View at Scopus
  32. K. Imaizumi, “Diet and atherosclerosis in apolipoprotein E-deficient mice,” Bioscience, Biotechnology and Biochemistry, vol. 75, no. 6, pp. 1023–1035, 2011. View at Publisher · View at Google Scholar · View at Scopus
  33. C. J. Vaughan and A. M. Gotto Jr., “Update on statins: 2003,” Circulation, vol. 110, no. 7, pp. 886–892, 2004. View at Publisher · View at Google Scholar · View at Scopus
  34. R. Jatwa and A. Kar, “Amelioration of metformin-induced hypothyroidism by Withania somnifera and Bauhinia purpurea extracts in type 2 diabetic mice,” Phytotherapy Research, vol. 23, no. 8, pp. 1140–1145, 2009. View at Publisher · View at Google Scholar · View at Scopus
  35. A. K. Singh, J. Joshi, and R. Jatwa, “Dipeptidyl peptidase IV (DPP-IV/CD26) inhibitory and free radical scavenging potential of W. somnifera and T. foenum-graecum extract,” International Journal of Phytomedicine, vol. 5, pp. 503–509, 2013. View at Google Scholar
  36. A. K. Singh, R. Jatwa, and J. Joshi, “Cytoprotective and dipeptidyl peptidase IV (DPP-IV/CD26) inhibitory roles of Ocimum sanctum and Momordica charantia extract,” Asian Journal of Pharmaceutical and Clinical Research, vol. 7, pp. 115–120, 2014. View at Google Scholar