Research Article

Development of Monoclonal Antibodies against CMP-N-Acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1 (ST3Gal-I) Recombinant Protein Expressed in E. coli

Figure 4

Western blot analysis of ST3Gal-I with 7E5 1C8 3A10 monoclonal antibodies. Lane 1: protein molecular weight marker from NEB; lane 2: purified recombinant ST3Gal-I protein (3 μg); lane 3: negative control 1 (Human Serum); lane 4: negative control 2 (recombinant galectin 3 containing 6x His tag, 31.5 kDa).