Influenza A Virus and Influenza B Virus Can Induce Apoptosis via Intrinsic or Extrinsic Pathways and Also via NF-κB in a Time and Dose Dependent Manner

Table 2

Viral and cellular genes expression levels in response to H3N2 influenza virus of A549 and MDCK cells evaluated by quantitative RT-PCR at the indicated time point. Ct values were determined for caspases 3, 8, and 9, TNFα, IκBα, BAD, MxA, and -actin and GPDH genes.

H3N2	IκBα		BAD		TNF		Caspase 9		Caspase 8		Caspase 3		MXA		-actin		GPDH
	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0
MDCK
16 hpi	22.46	22.76	16.38	15.85	25	25.8	24.2	25.3	25	26	26	276	27	26	17.2	17.2	22.2	22.2
24 hpi	26.57	26.33	19.77	20.98	28.4	28.79	27.6	28.4	29	30	31.2	30.8	28	29	17.5	17.6	23.6	23.6
36 hpi	24.82	25.2	18.4	19.9	26.72	26.99	26.4	27.0	27	27	28.7	27.4	26	28	17.9	18	23.2	23.2
48 hpi	23.2	23.22	24.66	27.75	25.9	25.9	25.69	27	26	26	26.9	26.4	26	26	18.3	18.4	23	23
A549
16 hpi	19.2	19.69	16.8	18.4	20	20.9	22.8	21.9	19	20.6	21	21.6	27	27	16.9	16.9	20.3	20.6
24 hpi	22.63	22.75	17.55	17.66	23	22.7	23	22.7	23.2	24.1	25.2	25.2	31	31.66	17.2	17.2	21.0	21.0
36 hpi	21.25	22.25	18.22	22.5	24	22.9	24	23.6	21.65	21.45	23	23	29.4	29.88	17.9	17.8	21.0	21.0
48 hpi	19.36	20.15	17.2	17.95	22	21.4	21.77	22.2	21	19.8	21.9	21.88	27.4	27.89	17.2	17.0	21.0	21.0