Table 2: Viral and cellular genes expression levels in response to H3N2 influenza virus of A549 and MDCK cells evaluated by quantitative RT-PCR at the indicated time point. Ct values were determined for caspases 3, 8, and 9, TNFα, IκBα, BAD, MxA, and -actin and GPDH genes.

H3N2 IκBαBADTNFCaspase 9Caspase 8Caspase 3MXA-actinGPDH
MOI 0.1MOI 1.0MOI 0.1MOI 1.0MOI 0.1MOI 1.0MOI 0.1MOI 1.0MOI 0.1MOI 1.0MOI 0.1MOI 1.0MOI 0.1MOI 1.0MOI 0.1MOI 1.0MOI 0.1MOI 1.0

MDCK
 16 hpi22.4622.7616.3815.852525.824.225.3252626276272617.217.222.222.2
 24 hpi26.5726.3319.7720.9828.428.7927.628.4293031.230.8282917.517.623.623.6
 36 hpi24.8225.218.419.926.7226.9926.427.0272728.727.4262817.91823.223.2
 48 hpi23.223.2224.6627.7525.925.925.6927262626.926.4262618.318.42323
A549
 16 hpi19.219.6916.818.42020.922.821.91920.62121.6272716.916.920.320.6
 24 hpi22.6322.7517.5517.662322.72322.723.224.125.225.23131.6617.217.221.021.0
 36 hpi21.2522.2518.2222.52422.92423.621.6521.45232329.429.8817.917.821.021.0
 48 hpi19.3620.1517.217.952221.421.7722.22119.821.921.8827.427.8917.217.021.021.0