Influenza A Virus and Influenza B Virus Can Induce Apoptosis via Intrinsic or Extrinsic Pathways and Also via NF-κB in a Time and Dose Dependent Manner

Table 3

Viral and cellular genes expression levels in response to influenza B virus of A549 and MDCK cells evaluated by quantitative RT-PCR at the indicated time point. Ct values were determined for caspases 3, 8, and 9, TNFα, IκBα, BAD, MxA, and -actin, and GPDH genes.

INF B	IκBα		BAD		TNF		Caspase 9		Caspase 8		Caspase 3		MXA		β actin		GPDH
	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0
MDCK
16 hpi	16	16.76	18.38	18.85	20	20.4	23.2	24.3	—	—	16.8	16.6	22	23	17.2	17.2	22	22.2
24 hpi	20.57	20.33	22.77	22.9	24.63	24.89	26.6	27.4	—	—	21.2	20.8	26	26.7	17.6	17.6	23.5	23.6
36 hpi	18.82	18.63	20.4	20.9	22.78	23.16	25.4	26.0	—	—	18.7	17.4	24.2	24.5	18	18	23.0	23.2
48 hpi	17.2	17.22	19.66	19.75	20.9	20.9	24.6	25.9	—	—	16.9	17.4	22.7	22.9	18.4	18.4	23.1	23
A549
16 hpi	18.2	18.44	19.8	18.4	19.4	19.9	—	—	19.8	20.6	15.9	15.9	19	27	16.9	16.9	21	20.66
24 hpi	22.63	22.75	23.55	24.1	22	21.7	—	—	22.2	23.1	19.2	19.2	22	22.7	17.4	17.2	21	21.0
36 hpi	20.25	20.6	21.2	21.7	21.2	21.9	—	—	21.65	21.45	17	17	20.4	20.8	17.9	17.8	21.3	21.0
48 hpi	18.9	19.15	20	20	20.7	20.2	—	—	21.4	19.6	16.9	16.4	19.4	19.6	17.2	17.0	21.1	21.0

Undetectable or very low expression level.