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Biochemistry Research International
Volume 2016, Article ID 6025245, 8 pages
http://dx.doi.org/10.1155/2016/6025245
Research Article

Modulation of Erythrocyte Plasma Membrane Redox System Activity by Curcumin

1Department of Biochemistry, University of Allahabad, Allahabad 211002, India
2Division of Applied Science & Indo-Russian Center For Biotechnology (IRCB), Indian Institute of Information Technology, Allahabad 211012, India

Received 17 October 2015; Revised 30 December 2015; Accepted 31 December 2015

Academic Editor: David L. Daleke

Copyright © 2016 Prabhakar Singh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Plasma membrane redox system (PMRS) is an electron transport chain system ubiquitously present throughout all cell types. It transfers electron from intracellular substrates to extracellular acceptors for regulation of redox status. Curcumin, isolated from Curcuma longa, has modulatory effects on cellular physiology due to its membrane interaction ability and antioxidant potential. The present study investigates the effect of curcumin on PMRS activity of erythrocytes isolated from Wistar rats in vitro and in vivo and validated through an in silico docking simulation study using Molegro Virtual Docker (MVD). Effects of curcumin were also evaluated on level of glutathione (GSH) and the oxidant potential of plasma measured in terms of plasma ferric equivalent oxidative potentials (PFEOP). Results show that curcumin significantly () downregulated the PMRS activity in a dose-dependent manner. Molecular docking results suggest that curcumin interacts with amino acids at the active site cavity of cytochrome reductase, a key constituent of PMRS. Curcumin also increased the GSH level in erythrocytes and plasma while simultaneously decreasing the oxidant potential (PFEOP) of plasma. Altered PMRS activity and redox status are associated with the pathophysiology of several health complications including aging and diabetes; hence, the above finding may explain part of the role of curcumin in health beneficial effects.