Purification and Characterization of a Novel Intracellular Sucrase Enzyme of Leishmania donovani Promastigotes

Figure 4

The temperature tolerance and optimum pH of intra- and extracellular sucrase: (a) the intra- and extracellular enzymes were preincubated for 30 min at different temperature (4–60°C) prior to estimating the enzyme activity under standard assay condition in 50 mM sodium acetate buffer at pH 6.0 and pH 5.5, respectively. The enzyme activities of intracellular (--) and extracellular sucrase (--) were plotted as percentage of maximum activity (100%) at different temperature. (b) The pH optimum was measured by performing activity assay at different pH at a range of 02.75–8.0 as mentioned in Materials and Methods. The intracellular (--) and extracellular (--) sucrase activities were plotted as a percentage of activity versus pH, with maximum activity being 100%. Each point represents the mean of three different experiments.

(a)  

(b)