Research Article

Purification and Characterization of a Novel Intracellular Sucrase Enzyme of Leishmania donovani Promastigotes

Table 1

Purification of intracellular sucrase: the table shows the list of different steps, taken during the purification process. The total enzyme activity, the amount of protein recovered, and the specific activity of the sucrase enzyme were estimated from each step of the purification procedure to calculate the increased fold of purification.

Steps of purificationTotal activity nmoles of glucose formed/minTotal protein µgSpecific activity nmoles of glucose/mg/minFold purification

Crude extract208479000226.371
Size-exclusion chromatography 10751107897.033.67
IEC-CM-cellulose854.363628235.548.93
IEC-DEAE Sephadex459.08125036713.92
SEC-Sephacryl S-200154.6434.544477.12169.78
Hydrophobic chromatography (HA batch absorption) 103.8611.8168789.77333.32