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Biochemistry Research International
Volume 2017, Article ID 6257240, 10 pages
Research Article

Synthesis and Evaluation of In Vitro Antibacterial and Antitumor Activities of Novel N,N-Disubstituted Schiff Bases

1Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100043, China
2State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guizhou 550014, China
3Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guizhou 550014, China
4Guizhou Medical University, Guizhou 550025, China

Correspondence should be addressed to Wei-dong Pan; moc.361@napdw and Xiao-dong Zhang; ten.362@185dxz

Received 29 November 2016; Revised 8 February 2017; Accepted 20 April 2017; Published 21 June 2017

Academic Editor: Tzi Bun Ng

Copyright © 2017 Heng Luo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


To get inside the properties of N,N-disubstituted Schiff bases, we synthesized three high-yielding benzaldehyde Schiff bases. We used the reaction between salicylaldehyde and different diamine compounds, including diamine, ethanediamine, and o-phenylenediamine, determining the structure of obtained molecules by nuclear magnetic resonance spectroscopy and electrospray ionization mass spectroscopy. We thus evaluated the microbicidal and antitumor activity of these compounds, showing that salicylaldehyde-hydrazine hydrate Schiff base (compound 1a) significantly inhibited the growth of S. aureus; salicylaldehyde-o-phenylenediamine Schiff base (compound 1c) displayed a strong capability to inhibit the proliferation of leukemia cell lines K562 and HEL. Moreover, we observed that the antibacterial action of 1a might be associated with the regulation of the expression of key virulence genes in S. aureus. Compound 1c resulted in a strong apoptotic activity against leukemia cells, also affecting the cell cycle distribution. Overall, our novel N,N-disubstituted Schiff bases possess unique antibacterial or antitumor activities that exhibit the potent application prospect in prophylactic or therapeutic interventions, providing new insights for developing new antibacterial and anticancer chemical agents.