Synthesis and Evaluation of In Vitro Antibacterial and Antitumor Activities of Novel N,N-Disubstituted Schiff Bases

Table 2

IC₅₀ values of compounds 1a, 1b, and 1c on cancer cell lines.

Cancer cell lines	IC50 (μmol/L)
	1a	1b	1c	Adriamycin
PC3	131.26 ± 15.36	59.78 ± 12.13	67.24 ± 13.14	8.06 ± 1.42
BPH1	214.61 ± 14.25	90.03 ± 11.36	67.39 ± 15.89	11.36 ± 1.14
K562	—	52.22 ± 10.39	11.95 ± 2.36	4.56 ± 0.88
HEL	—	—	9.72 ± 2.56	3.12 ± 0.32

Cell lines include the following: human prostate cancer cell line (PC3), human prostate mesenchymal cell line (BPH1), and human leukemia cell lines (K562 and HEL). Cell viability (%) was determined by MTT assay to calculate the IC₅₀. All the assayed compounds were dissolved in DMSO, with a final concentration of DMSO was less than 0.1%. Control cells were treated only with the medium containing 0.1% DMSO. Values were mean ± standard deviation of three independent experiments.