Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex

Figure 1

Multiple-sequence alignments of Med6p homologs. Sequences of Med6 proteins from S. cerevisiae (scMed6), S. pombe (spMed6), C. elegans (cMED6), D. melanogaster (dMed6), and H. sapiens (hMED6) are aligned with the aid of Expresso (Di Tommaso et al. [24]) and presented by ESPript 3.0 program (Robert and Gouet [25]) along with the secondary structure information of spMed6 (pdb code 4H63 and 5N9J). Black and shaded boxes indicate identical and similar amino acids, respectively, and gaps are represented as dots. Ten regions chosen for the internal deletion (10 amino acids each) are also shown as thick lines just below the corresponding amino acid sequences of scMed6p. The mutational sites found in med6-ts1 and med6-ts2 alleles are depicted as sharp (#) and asterisk (∗), respectively.