Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex

Figure 3

Identification of the regions in Med6p required for cell viability. (a) Schematic depiction of plasmid shuffle technique for the complementation test of mutant MED6 genes (med6Δ). (b) The growth complementation test of the med6 deletion mutants. Wild-type (WT) MED6 and indicated med6 deletion mutants in the pRS313 vector were introduced into the host strain YCL4 (med6Δ::LEU2 and pRS316-MED6) for plasmid shuffling. The resulting transformants were grown for 4 days at 30°C on synthetic complete media containing 5-fluoroorotic acid to remove the pRS316-MED6. (–): pRS313 vector only.

(a)

(b)